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Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Frontiers of Agricultural Science and Engineering 2014, Volume 1, Issue 3,   Pages 179-184 doi: 10.15302/J-FASE-2014020

Abstract: Due to the limited yield of human BCHE (hBCHE) when purifying from human plasma, it is necessary to findIn this study, we cloned the gene into a human lactoferrin (hLF) bacterial artificial chromosome (BAC) construct to make a hLF-hBCHE BAC construct.Subsequently, we injected the BAC construct into pronuclei of mouse fertilized embryos and generated

Keywords： recombinant human butyrylcholinesterase (rhBCHE)     human lactoferrin bacterial artificial chromosome (hLFBAC)     transgenic mice     milk    

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A bacterial artificial chromosome-based physical map of

Yuhua FU,Zhiqiang XIA,Shujuan WANG,Xin CHEN,Cheng LU,Mingcheng LUO,Hongbin ZHANG,Wenquan WANG

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 4,   Pages 321-329 doi: 10.15302/J-FASE-2016124

Abstract: Two new bacterial artificial chromosome (BAC) libraries were constructed for W14 ( ssp. ), a wild ancestorsingletons, representing approximately 762 Mb of the cassava genome. 5000 clones located at the ends of BACThis BAC-based physical map provides valuable tools to understand the genetics and evolution of cassava

Keywords： cassava     BAC library     physical map    

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Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC

Shuting ZHANG, Bo WEI, Xin YU, Bing LIU, Zhuoying WU, Li GU

Frontiers of Environmental Science & Engineering 2010, Volume 4, Issue 4,   Pages 459-465 doi: 10.1007/s11783-010-0249-3

Abstract: The biologic activated carbon (BAC) process is widely used in drinking water treatments.However, the bottleneck of deoxyribonucleic acid (DNA) extraction from BAC attached biofilm has to beconventional procedure was unsuccessful due to firm biomass attachment and adsorption capacity of the BACThis protocol was further tested using the vertical BAC samples from the full-scale biofilter of PinghuThe results showed the DNA yielded a range of 40 μg·g BAC (dry weight) to over 100 μg·g BAC (dry weight

Keywords： bacterial DNA extraction     biological activated carbon (BAC)     biofilm     water treatment     pretreatment protocol    

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Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Frontiers of Agricultural Science and Engineering 2018, Volume 5, Issue 3,   Pages 382-389 doi: 10.15302/J-FASE-2018211

Abstract: Our methodology is expected to have the advantages of both plasmid and bacterial artificial chromosome(BAC) vectors.The BAC of mouse whey acidic protein gene (mWAP) was modified twice by homologous recombinationto produce a universal expression vector, and the human lysozyme gene (hLZ) was then insertedfertilized mouse embryos, to successfully generate two transgenic mouse lines that expressed recombinant human

Keywords： BAC recombinant methods     gene expression     human lysozyme     transgenic mice     milk expression vector    

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Impact of preozonation on the bioactivity and biodiversity of subsequent biofilters under low temperature conditions—A pilot study

Jiaxuan YANG, Jun MA, Dan SONG, Xuedong ZHAI, Xiujuan KONG

Frontiers of Environmental Science & Engineering 2016, Volume 10, Issue 4, doi: 10.1007/s11783-016-0844-z

Abstract: The combination of preozonation and subsequent biological granular activated carbon (O /BAC) filtrationThe elevated functionality in O /BAC filters can be a result of increased microbial activity, which was

Keywords： Preozonation     BAC filtration     High ammonia level     Bacterial activity     Microbial community structure     Pilot    

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Transport of bacterial cell (

Wei Fan, Qi Li, Mingxin Huo, Xiaoyu Wang, Shanshan Lin

Frontiers of Environmental Science & Engineering 2020, Volume 14, Issue 4, doi: 10.1007/s11783-020-1242-0

Abstract: Commonly used recharge water resources for artificial groundwater recharge (AGR) such as secondary effluent

Keywords： Artificial groundwater recharge     E. coli     Transport     Simulated column experiments     Modeling    

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Artificial intelligence and statistics Perspective

Bin YU, Karl KUMBIER

Frontiers of Information Technology & Electronic Engineering 2018, Volume 19, Issue 1,   Pages 6-9 doi: 10.1631/FITEE.1700813

Abstract: Artificial intelligence (AI) is intrinsically data-driven.It calls for the application of statistical concepts through human-machine collaboration during the generationThis paper discusses how such human-machine collaboration can be approached through the statistical conceptsThe PQRS workflow provides a conceptual framework for integrating statistical ideas with human input

Keywords： Artificial intelligence     Statistics     Human-machine collaboration    

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The imperative need to develop guidelines to manage human versus machine intelligence

Donald KENNEDY, Simon P. PHILBIN

Frontiers of Engineering Management 2018, Volume 5, Issue 2,   Pages 182-194 doi: 10.15302/J-FEM-2018085

Abstract: Machine intelligence is increasingly entering roles that were until recently dominated by human intelligenceTherefore, this research explores the emerging area of human versus machine decision-making.An illustrative engineering case involving a joint machine and human decision-making system is presentedWe offer that the speed at which new human-machine interactions are being encountered by engineeringHuman-machine systems are becoming pervasive yet this research has revealed that current technological

Keywords： human intelligence & machine intelligence     HI-MI     decision-making     artificial intelligence    

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Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 3,   Pages 242-248 doi: 10.15302/J-FASE-2015059

Abstract: Lactoferrin is a member of the transferrin family of multifunctional iron binding glycoproteins.While numerous physiological functions have been described for lactoferrin, the mechanisms underlyingTo further study the functions and mechanisms of lactoferrin, we modified the lactoferrin promoter ofmice using the CRISPR/Cas9 system to reduce or eliminate lactoferrin expression.the plasmid pX330, expressing a small guide RNA and human codon-optimized SpCas9, into fertilized eggs

Keywords： lactoferrin     promoter     CRISPR/Cas9     plasmid pX330    

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Intelligence Originating from Human Beings and Expanding in Industry— A View on the Development of Artificial

Jiang Changjun, Wang Junli

Strategic Study of CAE 2018, Volume 20, Issue 6,   Pages 93-100 doi: 10.15302/J-SSCAE-2018.06.015

Abstract:

Artificial Intelligence (AI) aims to simulate information storage andprocessing mechanisms and other intelligent behaviors of a human brain, so that the machine has a certainhistorical integration and evolution of computer science, control science, brain-inspired intelligence, human

Keywords： artificial intelligence     human brain intelligence     brain-inspired intelligence     intelligence development    

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Philadelphia chromosome-positive acute myeloid leukemia with masses and osteolytic lesions: finding of

Zhan Su, Fengyu Wu, Weiyu Hu, Xiaodan Liu, Shaoling Wu, Xianqi Feng, Zhongguang Cui, Jie Yang, Zhenguang Wang, Hongzai Guan, Hongguo Zhao, Wei Wang, Chunting Zhao, Jun Peng

Frontiers of Medicine 2017, Volume 11, Issue 3,   Pages 440-444 doi: 10.1007/s11684-017-0523-x

Abstract:

Philadelphia chromosome-positive acute myeloid leukemia is controversial and difficult to distinguishFor the existence of Philadelphia chromosome and BCR-ABL1 fusion gene together with complex chromosome

Keywords： Philadelphia chromosome     acute myeloid leukemia     mass     osteolysis     positron emission tomography    

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prognostic factors of imatinib plus CALLG2008 protocol in adult patients with newly diagnosed Philadelphia chromosome-positive

Yinjun Lou, Yafang Ma, Chenyin Li, Sansan Suo, Hongyan Tong, Wenbin Qian, Wenyuan Mai, Haitao Meng, Wenjuan Yu, Liping Mao, Juyin Wei, Weilei Xu, Jie Jin

Frontiers of Medicine 2017, Volume 11, Issue 2,   Pages 229-238 doi: 10.1007/s11684-017-0506-y

Abstract: We retrospectively analyzed 153 newly diagnosed adult patients with Philadelphia chromosome (Ph)-positive

Keywords： Philadelphia chromosome     acute lymphoblastic leukemia     imatinib     CALLG2008    

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Heading toward Artificial Intelligence 2.0

Yunhe Pan

Engineering 2016, Volume 2, Issue 4,   Pages 409-413 doi: 10.1016/J.ENG.2016.04.018

Abstract: in size of the information community, and interlinking and fusion of data and information throughout human, physical space, and cyberspace, the information environment related to the current development of artificial

Keywords： Artificial intelligence 2.0     Big data     Crowd intelligence     Cross-media     Human-machine     hybrid-augmented     intelligence    

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The predictive value of chromosome 8p deletion for metastasis of hepatocellular carcinoma: a summary

QIN Lunxiu, TANG Zhaoyou, GUAN Xinyuan, YE Qinghai, JIA Huliang, REN Ning

Frontiers of Medicine 2008, Volume 2, Issue 3,   Pages 211-215 doi: 10.1007/s11684-008-0041-y

Abstract: the genomic aberrations of the clinical specimens, as well as the metastatic models and cell lines of humanchromosomal aberrations between primary HCC tumors and their matched metastatic lesions, and found that chromosome

Keywords： sensitive genome-wide     prediction     genome-wide microsatellite     frequency     Hepatocellular carcinoma    

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Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 3, doi: 10.1007/s11783-021-1466-7

Abstract:

• The α-diversities of resistome were lower in manure and compost than in soils.

Keywords： Antibiotic resistance genes     Resistome     Bacterial taxonomy     Transmission chain    

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Title Author Date Type Operation

Expression of recombinant human butyrylcholinesterase in the milk of transgenic mice

Dan LU,Shengzhe SHANG,Shen LIU,Ying WU,Fangfang WU,Tan TAN,Qiuyan LI,Yunping DAI,Xiaoxiang HU,Yaofeng ZHAO,Ning LI

Journal Article

A bacterial artificial chromosome-based physical map of

Yuhua FU,Zhiqiang XIA,Shujuan WANG,Xin CHEN,Cheng LU,Mingcheng LUO,Hongbin ZHANG,Wenquan WANG

Journal Article

Development of pretreatment protocol for DNA extraction from biofilm attached to biologic activated carbon (BAC

Shuting ZHANG, Bo WEI, Xin YU, Bing LIU, Zhuoying WU, Li GU

Journal Article

Construction of a universal recombinant expression vector that regulates the expression of human lysozyme

Shen LIU, Shengzhe SHANG, Xuezhen YANG, Huihua ZHANG, Dan LU, Ning LI

Journal Article

Impact of preozonation on the bioactivity and biodiversity of subsequent biofilters under low temperature conditions—A pilot study

Jiaxuan YANG, Jun MA, Dan SONG, Xuedong ZHAI, Xiujuan KONG

Journal Article

Transport of bacterial cell (

Wei Fan, Qi Li, Mingxin Huo, Xiaoyu Wang, Shanshan Lin

Journal Article

Artificial intelligence and statistics

Bin YU, Karl KUMBIER

Journal Article

The imperative need to develop guidelines to manage human versus machine intelligence

Donald KENNEDY, Simon P. PHILBIN

Journal Article

Generation of CRISPR/Cas9-mediated lactoferrin-targeted mice by pronuclear injection of plasmid pX330

Mengxu GE,Fei LIU,Fei CHANG,Zhaolin SUN,Jing FEI,Ying GUO,Yunping DAI,Zhengquan YU,Yaofeng ZHAO,Ning LI,Qingyong MENG

Journal Article

Intelligence Originating from Human Beings and Expanding in Industry— A View on the Development of Artificial

Jiang Changjun, Wang Junli

Journal Article

Philadelphia chromosome-positive acute myeloid leukemia with masses and osteolytic lesions: finding of

Zhan Su, Fengyu Wu, Weiyu Hu, Xiaodan Liu, Shaoling Wu, Xianqi Feng, Zhongguang Cui, Jie Yang, Zhenguang Wang, Hongzai Guan, Hongguo Zhao, Wei Wang, Chunting Zhao, Jun Peng

Journal Article

prognostic factors of imatinib plus CALLG2008 protocol in adult patients with newly diagnosed Philadelphia chromosome-positive

Yinjun Lou, Yafang Ma, Chenyin Li, Sansan Suo, Hongyan Tong, Wenbin Qian, Wenyuan Mai, Haitao Meng, Wenjuan Yu, Liping Mao, Juyin Wei, Weilei Xu, Jie Jin

Journal Article

Heading toward Artificial Intelligence 2.0

Yunhe Pan

Journal Article

The predictive value of chromosome 8p deletion for metastasis of hepatocellular carcinoma: a summary

QIN Lunxiu, TANG Zhaoyou, GUAN Xinyuan, YE Qinghai, JIA Huliang, REN Ning

Journal Article

Antibiotic resistome mostly relates to bacterial taxonomy along a suburban transmission chain

Journal Article